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15 Flashcards in this deck.
DNA, or Deoxyribonucleic Acid, is a long molecule that contains the genetic instructions used in the growth, development, functioning, and reproduction of all known living organisms and many viruses. It is the carrier of genetic information and is essential for heredity.
The structure of DNA is famously known as a double helix, resembling a twisted ladder. This structure was first described by James Watson and Francis Crick in 1953, based on the X-ray diffraction images taken by Rosalind Franklin.
The double helix consists of two strands of nucleotides wound around each other. Each nucleotide is composed of three components:
The nitrogenous bases pair specifically through hydrogen bonds, following the base-pairing rules:
This specific pairing ensures accurate replication of genetic information during cell division.
The two strands of DNA run in opposite directions, a characteristic known as antiparallel. One strand runs in a 5’ to 3’ direction, while the other runs 3’ to 5’. This orientation is crucial for the replication and transcription processes.
The twisting of the DNA strands creates major and minor grooves. These grooves serve as binding sites for proteins and other molecules that regulate gene expression and replication.
DNA wraps around histone proteins to form nucleosomes, which further coil to form chromatin. This packaging allows DNA to fit within the cell nucleus and plays a role in gene regulation.
DNA replication is the process by which DNA makes a copy of itself during cell division. It involves unwinding the double helix, pairing of complementary bases, and synthesis of new strands by DNA polymerase enzymes.
Replication is semi-conservative, meaning each new DNA molecule consists of one original strand and one newly synthesized strand.
$$ \text{Semi-conservative Replication:} \quad \text{DNA}_\text{original} \rightarrow \text{DNA}_\text{new} = \text{Original Strand} + \text{New Strand} $$
The sequence of nitrogenous bases in DNA constitutes the genetic code, which dictates the synthesis of proteins. This code is read in triplet codons during the process of translation.
DNA is organized into structures called chromosomes. Humans have 23 pairs of chromosomes, which carry genes that determine individual traits and hereditary characteristics.
Gene expression involves the transcription of DNA into messenger RNA (mRNA) and the translation of mRNA into proteins. This process is tightly regulated to ensure proper cellular function.
Mutations are changes in the DNA sequence. They can occur due to errors during replication or exposure to mutagens. While some mutations are harmless, others can lead to genetic disorders or contribute to evolution.
Advancements in DNA technology, such as Polymerase Chain Reaction (PCR), Genetic Engineering, and Genome Sequencing, have revolutionized fields like medicine, forensics, and biotechnology.
Understanding DNA structure and function has numerous applications, including:
Despite significant advancements, DNA research faces challenges such as ethical concerns in genetic engineering, data privacy in genetic information, and the complexity of genetic interactions influencing traits.
Aspect | DNA | RNA |
---|---|---|
Structure | Double helix | Single-stranded |
Sugar | Deoxyribose | Ribose |
Nitrogenous Bases | Adenine (A), Thymine (T), Cytosine (C), Guanine (G) | Adenine (A), Uracil (U), Cytosine (C), Guanine (G) |
Function | Stores genetic information | Involved in protein synthesis |
Location | Nucleus (in eukaryotes) | Nucleus and Cytoplasm |
Stability | More stable | Less stable |
1. **Use Mnemonics:** To remember base pairing, use "A-T and G-C" or "All Twins Get Clones."
2. **Visual Aids:** Draw the double helix and label the components to reinforce structural understanding.
3. **Practice Diagrams:** Regularly sketch DNA replication and transcription processes to enhance retention.
4. **Connect Concepts:** Relate DNA structure to its functions in heredity and protein synthesis for deeper comprehension.
1. DNA molecules are incredibly long; if stretched out, the DNA from a single human cell would be about 2 meters in length.
2. Despite its length, all the DNA in your body fits into a nucleus that is just a few micrometers in diameter.
3. The discovery of DNA's structure has led to groundbreaking medical advancements, including the development of personalized medicine tailored to an individual's genetic makeup.
Incorrect: Confusing RNA with DNA structure.
Correct: Remember that DNA is double-stranded with thymine, while RNA is single-stranded with uracil.
Incorrect: Misplacing the 5’ and 3’ ends during replication.
Correct: Always ensure that DNA strands are antiparallel, with one strand running 5’ to 3’ and the other 3’ to 5’.
Incorrect: Overlooking the role of histones in DNA packaging.
Correct: Understand that histones help in organizing and compacting DNA into chromatin, facilitating gene regulation.