AS & A Level Biology - 9700 Paper 1 2022 Winter Zone 3

The electron micrograph shows a chloroplast from a tobacco leaf.
If the actual length of this chloroplast measured along X–Y is 10 $\mu$m, what is the magnification of the image?

Some cell structures are listed.
1. endoplasmic reticulum
2. Golgi body
3. mitochondrion
4. chloroplast
Which cell structures do not contain cristae?

Which letter represents cell structures where mRNA may be found?

Which row is correct for typical prokaryotic cells and mitochondria?
[Table_1]

Which statement is correct?

The Benedict’s test and ethanol emulsion test were carried out on a sample of biological molecules. The solution became brick-red during the Benedict’s test and cloudy during the ethanol emulsion test.
Which molecules did the sample contain?

Which diagram is correct for $\alpha$-glucose?

Which reaction correctly represents one way sucrose can form?

The diagram represents part of an alginic acid molecule. Alginic acid is a polymer found in the cell walls of brown seaweeds.

Which statement about alginic acid is correct?

Which features are correct for triglycerides and phospholipids?
1 They have ester bonds between glycerol and fatty acids.
2 Fatty acid chains may have single or double bonds between carbon atoms in the chains.
3 The fatty acid chains are non-polar and hydrophobic.
4 Saturated fatty acid chains allow closer packing of the molecules than unsaturated fatty acid chains.

A scientist studying the structure of a protein reported that it consists of two polypeptide chains joined by disulfide bonds. Which feature of protein structure does this describe?

Some molecules fluoresce when illuminated with ultraviolet light. Changes in the structure or shape of such molecules can change the intensity of the fluorescence.

The enzyme tryptophan synthase fluoresces bluish-green because of a phosphate group associated with the active site. The enzyme catalyses the reaction shown.

$$ ext{serine + indole} ightarrow ext{tryptophan}$$

Adding serine to the enzyme increases the intensity of the fluorescence, but when indole is also added the bluish-green fluorescence decreases in intensity.

What may be concluded from these observations?
1 Serine and indole attach to the active site of the enzyme.
2 An enzyme–serine complex is formed.
3 An enzyme–serine–indole complex is formed.

Yeast contains the enzyme catalase which catalyses the breakdown of hydrogen peroxide (H2O2) as shown.

Yeast was added to a solution of hydrogen peroxide and the total volume of oxygen released was recorded every 30 seconds for 2 minutes. All other variables were standardised.
The data is shown in the table.
[Table_1]
What explains the pattern of the data?

The Michaelis–Menten constant, $K_m$, is a measure of the affinity of an enzyme for its substrate.
1. The higher the affinity, the lower the $K_m$.
2. The lower the affinity, the slower the reaction will be.
3. At $K_m$, half the active sites of the enzyme are occupied by the substrate.
Which statements about $K_m$ are correct?

Which features are correct for active transport and facilitated diffusion?
1. The movement of molecules and ions depends on ATP.
2. They are specific for one type of molecule or ion.
3. They use membrane proteins.

The diagram represents two solutions, P and Q, that were separated by a partially permeable dialysis tubing.
[Image_1: Diagram showing solutions P and Q separated by a partially permeable dialysis tubing]
What will be the initial movement of the molecules, 1, 2 and 3, between solution P and solution Q?

A student weighed a cylinder of potato and then put it into a test-tube containing a salt solution.
The potato cylinder was removed from the salt solution after one hour. It was blotted dry and then reweighed. The student recorded that the potato had lost mass.
Which row shows the correct explanation for the results the student collected?
[Table_1]

Which row shows the correct number of each component in a single chromosome at the start of telophase of mitosis? [Table_1]

The photomicrographs show cells in various stages of the cell cycle.
In which stage does semi-conservative replication of DNA take place?

The diagram shows the epidermis of human skin. This is formed of four layers of cells.

Cells in each layer have specific functions which are outlined on the diagram. As the cells are produced, they move upwards as shown by the arrow.

Which row shows cells that are acting as stem cells in the skin epidermis and an organelle needed by the keratinocytes?

The diagram shows some of the stages that take place during the cell cycle.

Which two stages take place during interphase?

What does a nucleotide contain?
1 an amino acid
2 a codon
3 a nitrogenous base
4 a sugar

A diagram can be used to show some relationships between different nucleic acid bases.

Which row is correct for the words that can be placed at positions 1–5?

The bacterium Escherichia coli divides once every 50 minutes at 36°C.
E. coli were grown on a medium containing only heavy nitrogen, $^{15}N$, until all of the bacterial DNA contained heavy nitrogen.
Some of the bacteria were moved from a heavy nitrogen medium and cultured in a medium with only light nitrogen, $^{14}N$ (0 minutes).
These bacteria continued to reproduce and samples were extracted and centrifuged at regular intervals.
Hybrid DNA contains both heavy and light nitrogen.
The diagram shows the possible positions (upper, middle and lower) of the bands of DNA. The actual positions of bands in the first two samples are shown.

Which proportion of the DNA of the sample taken at 150 minutes will be at the upper position?

Following translation, the alpha polypeptide chain of haemoglobin, \( \alpha\)-globin, undergoes modification. During this modification, the first amino acid is removed, leaving 141 amino acid residues.
How many nucleotides does the mRNA coding for \( \alpha\)-globin contain?

The photomicrograph shows a section through a plant organ.
Which statement could be used to describe this organ?

The statements describe some of the properties of water.
1. requires a lot of heat energy to evaporate
2. retains a lot of heat energy
3. is able to form hydrogen bonds with other water molecules
4. is able to form hydrogen bonds with other polar and non-polar molecules
Which properties are also important for transport in xylem?

Which xerophytic adaptations reduce the water potential gradient between leaf surface and atmosphere?
1. rolled leaves
2. hairy leaves
3. sunken stomata
4. fewer stomata
5. fleshy leaves

The diagram shows seven stages of a tulip bulb’s growth.
Which row identifies the correct stages in which the tulip bulb is acting as a source only or as a sink only?
[Table_1]

What would be changed if mitochondrial activity was inhibited by a metabolic poison acting on cells in the phloem tissue?
1 concentration of hydrogen ions in the cell wall of companion cells
2 concentration of sucrose in the cytoplasm of cells in a leaf
3 hydrostatic pressure gradient in the phloem sieve tube

By which process does sucrose move through phloem sieve tube elements?

Which blood vessels carry blood into the atria?
1 coronary artery
2 vena cava
3 pulmonary artery
4 pulmonary vein

Which statements about the cardiac cycle are correct?
1. The atrioventricular valves close during atrial systole.
2. The left and right ventricles contract together.
3. There is a time delay as the electrical impulse passes from the atrioventricular node to the sinoatrial node.
4. Electrical impulses pass along the Purkyne tissue to the ventricles.

At one point in the cardiac cycle, the pressure in the right ventricle is lower than that in the right atrium and lower than that in the pulmonary artery.
Which row is correct?
[Table_1]

Which statements about the formation of haemoglobinic acid are correct?
1. It is linked to the action of carbonic anhydrase.
2. It prevents blood from becoming too acidic by removing excess hydrogen ions.
3. It can only occur when oxygen associates with haemoglobin.

The graph shows the dissociation curves for haemoglobin at two different partial pressures of carbon dioxide. At which position on the graph would most chloride ions be found in the red blood cells?

Oedema is caused by the accumulation of tissue fluid around body cells. What would increase the amount of tissue fluid around body cells?

Certain bacteria develop resistance to an antibiotic by actively transporting the antibiotic out of the cell. This prevents the antibiotic building up to toxic concentrations.
What must be found in a bacterial cell to allow it to develop this form of antibiotic resistance?

Which statements explain the differences between a primary and a secondary response to exposure to the same antigen?
1. The primary response is slow, because there are only a few B-lymphocytes that are specific to the antigen.
2. The secondary response is faster, because there are many memory cells specific to the antigen.
3. More antibodies are produced during the secondary response, because many of the memory cells differentiate into plasma cells specific to the antigen.

Which statement about B-lymphocytes and T-lymphocytes is correct?