AS & A Level Biology - 9700 Paper 4 2023 Winter Zone 2

(a) Some organisms carry out respiration in anaerobic conditions when oxygen is not available or when there is a low concentration of oxygen. In yeast and some plants, this is called ethanol fermentation. In mammals, it is called lactate fermentation.

Fig. 1.1 outlines ethanol fermentation and lactate fermentation.



Identify substances A–D.
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D .................................................................................................................... [3]

(b) Explain how processes such as ethanol fermentation and lactate fermentation allow cells to continue to function in the absence of oxygen.
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(c) The cereal crop rice, Oryza sativa, grows in fields that are flooded with water. The roots of the rice plants are submerged in water that contains very little oxygen.

Describe and explain how rice plants are adapted to grow in flooded fields.
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(a) With reference to Fig. 2.1, state the numbers of the stages 1–5 that:

involve mitosis ....................
involve meiosis ....................
produces new genetic variation ....................
shows only haploid cells ....................
shows only diploid cells ....................
[Image_2.1]

(b) When there is a lack of nutrients, cells made in stage 3 will carry out stage 4 to make spores,
which germinate only when conditions improve.

Suggest and explain how the type of reproduction that makes spores during stage 4 is advantageous
for S. cerevisiae in a changing environment.
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(c) Haploid and diploid cells of S. cerevisiae can carry out asexual reproduction.

Suggest why a new harmful recessive mutation may not have a damaging effect on:
● an asexually reproducing population of haploid cells of S. cerevisiae
● an asexually reproducing population of diploid cells of S. cerevisiae.
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(d) State two features, other than reproduction using spores, of the kingdom Fungi.
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Research suggests that selective breeding began about 10 000 years ago. Selective breeding has been important in satisfying the global demand for food.

(a) The introduction of disease resistance to varieties of wheat is an example of selective breeding. Outline the steps involved in the introduction of disease resistance to varieties of wheat.

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(b) Suggest two possible disadvantages of selective breeding.

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(a) A marker gene can be used when a gene of interest is introduced into a plant by genetic engineering. Describe and explain how the use of a marker gene coding for a fluorescent product can show that the introduced gene of interest is being expressed in plants. [4]

(b) Give two advantages of genetically engineering herbicide resistance in crop plants such as soybean. [2]

(c) DNA microarray analysis is a technique used in genetic technology that involves fluorescence. A DNA microarray has single-stranded probes attached to its surface. These probes hybridise to the fluorescently tagged single-stranded DNA that is added.
(i) Explain why DNA hybridisation occurs between the probe DNA and the added DNA. [1]

(ii) A DNA microarray analysis can be used to identify the level of expression of some genes. Describe and explain how the level of expression of some genes can be identified using the DNA microarray analysis technique. [3]

The fruit fly, \textit{Drosophila melanogaster}, has autosomal genes for body colour and wing shape.
Gene $B/b$ is involved in the production of body colour:
• $B =$ dominant allele for brown body colour
• $b =$ recessive allele for black body colour.
Gene $D/d$ is involved in wing shape:
• $D =$ dominant allele for straight wing
• $d =$ recessive allele for curved wing.
A dihybrid test cross was carried out between flies heterozygous for body colour and for wing shape and flies homozygous recessive for body colour and for wing shape.
(a) Table 5.1 shows the number of offspring of each phenotype obtained in the test cross.
$$\text{Table 5.1}$$
$$\begin{array}{|c|c|c|} \hline \text{phenotype} & \text{observed number} & \text{expected number} \\ \hline \text{brown body colour, straight wings} & 2843 & \\ \text{brown body colour, curved wings} & 855 & \\ \text{black body colour, straight wings} & 842 & \\ \text{black body colour, curved wings} & 2768 & \\ \hline \end{array}$$
Use Table 5.1 to calculate the expected number of each phenotype if the two genes are on \textit{different} autosomes. Write your answers in the table. [1]
(b) A chi-squared ($\chi^2$) test was carried out to compare the observed results with the results that would be expected from a dihybrid cross involving genes on different autosomes.
The value of $\chi^2 = 2097.836$.
Table 5.2 shows the critical values for the $\chi^2$ distribution.
$$\text{Table 5.2}$$
$$\begin{array}{|c|c|c|c|} \hline \text{degrees of freedom} & p \text{ value} \ & 0.05 & 0.01 & 0.001 \\ \hline 1 & 3.841 & 6.635 & 10.828 \\ 2 & 5.991 & 9.210 & 13.816 \\ 3 & 7.815 & 11.345 & 16.266 \\ 4 & 9.488 & 13.277 & 18.467 \\ \hline \end{array}$$
Explain how the value of $\chi^2$ and Table 5.2 can be used to assess the significance of the difference between the observed results and the expected numbers in Table 5.1.
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(c) Suggest explanations for the \textbf{observed} results in Table 5.1.
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(a) (i) When blood glucose concentration decreases, glucagon is released by the pancreas into the blood and is transported to the cells.
Fig. 6.1 outlines the effect of glucagon on liver cells.

Fig. 6.1
Identify P, Q, R and S shown in Fig. 6.1.
P ...................................................................................................................................................
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(ii) Table 6.1 shows four processes carried out by liver cells that are affected by a decrease in blood glucose concentration. Some of these are cellular responses from cell signalling by glucagon.
Complete Table 6.1 by stating whether the rate of each process increases or decreases when blood glucose concentration decreases.
[Table_1]
Table 6.1

(b) Type 2 diabetes is a condition affecting the homeostatic control of blood glucose concentration.
In type 2 diabetes, target cells have a much lower response to insulin and some glucose is excreted in the urine.
Test strips can be used to measure the concentration of glucose in a sample of urine, but many people with type 2 diabetes measure blood glucose concentration using a biosensor.
Explain the principles of operation of a test strip for glucose and suggest advantages for a person with type 2 diabetes of using a biosensor instead of a test strip.
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(a) There are many mitochondria located in the presynaptic knobs of neuromuscular junctions and in the sarcomeres of muscle fibres of striated muscle.

Explain the need for many mitochondria in the presynaptic knobs and in the sarcomeres.

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(b) Fig. 7.1 is a diagram of synapses between a postsynaptic neurone and two presynaptic neurones, X and Y.



Neurone X releases the neurotransmitter acetylcholine.
Neurone Y releases the neurotransmitter glutamate.
Both neurotransmitters bind to channel proteins in the membrane of the postsynaptic neurone.
Acetylcholine binding results in an influx (entry) of sodium ions.
Glutamate binding results in an influx of chloride ions.

A student made three statements:
1. When only neurone X is stimulated, an action potential will occur in the postsynaptic neurone.
2. When only neurone Y is stimulated, an action potential will occur in the postsynaptic neurone.
3. When neurone X and neurone Y are stimulated at the same time, an action potential will not occur in the postsynaptic neurone.

Explain whether or not you agree with these statements.

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(c) Multiple sclerosis is a condition in which the myelin sheath breaks down in some neurones.

Suggest the effect of multiple sclerosis on the transmission of action potentials in the affected neurones.

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(a) Non-cyclic photophosphorylation occurs in the light-dependent stage of photosynthesis. Outline the main features of non-cyclic photophosphorylation. .......................................................................................................................................................................... .......................................................................................................................................................................... .......................................................................................................................................................................... .......................................................................................................................................................................... .......................................................................................................................................................................... .......................................................................................................................................................................... .......................................................................................................................................................................... .......................................................................................................................................................................... .......................................................................................................................................................................... .......................................................................................................................................................................... [4]

(b) Some Calvin cycle intermediates are used to produce other molecules that are \textbf{not} part of the Calvin cycle.
Name \textbf{two} of these intermediates.
For each intermediate, state \textbf{one} example of the type of molecule that is produced.

intermediate ................................................
molecule produced ................................................

intermediate ................................................
molecule produced ................................................ [2]

(c) Fig. 8.1 shows how the rate of uptake of carbon dioxide by a plant varies with temperature, at an optimum intensity of light on a clear day.
[Image_Fig_8.1]
(i) Calculate the mean increase in rate of uptake of carbon dioxide as the temperature increases from 5 °C to 20 °C.
Show your working.
Give your answer to \textbf{two} decimal places.
........................................... au °C^{-1} [2]
(ii) Suggest why the rate of carbon dioxide uptake levels off and then decreases after 20 °C.
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(iii) On Fig. 8.1, draw a curve to show the uptake of carbon dioxide on a cloudy day. [1]

(a) Antidiuretic hormone (ADH) is involved in the maintenance of a constant blood water potential.

A student drank 0.5 dm³ of water and the concentration of ADH in their blood was measured every 30 minutes for 3 hours.

Fig. 9.1 shows the results of this investigation.



Explain the results shown in Fig. 9.1.

(b) Outline the differences between the endocrine system and the nervous system.

(a) The passage outlines one method of estimating the size of an animal population. Complete the passage by using the most appropriate scientific terms.

The size of a population of animals can be estimated using the mark-release-recapture method. A sample of animals is captured using a humane (harmless) trap and is counted. The animals are marked using a method that does not harm them. This can be done using a small tag or ................................................ . The marked animals are released into the area where they were captured. Time is allowed for the marked animals to mix into the population. This period of time must be short so that emigration, immigration, migration or ................................................ do not occur.

A second sample of animals is then captured and the number of marked and unmarked animals is counted. The population size can be estimated using the ................................................ Index. For reliability, the method should be ................................................ .

(b) Himalayan balsam, Impatiens glandulifera, is an annual plant native to Pakistan, India and Nepal. It was first introduced into the United Kingdom (UK) in 1839 because of its attractive flowers. Individual plants can produce thousands of mature seeds. These can be dispersed for long distances when the capsules that contain the seeds burst.

Fig. 10.1 shows Himalayan balsam growing in a woodland.


Himalayan balsam has now become established in the UK. It is listed as an invasive alien species and attempts are being made to eradicate (remove) the plant.
Not all alien plant species are considered to be invasive.

Suggest why Himalayan balsam has been listed as an invasive alien species in the UK.
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